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    网赌时时彩惊天秘密:GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit

    日期: 2015-06-01
    浏览次数: 611

    河北十一选五走势图表 www.pmwk.net 货号:?A21175

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    规格

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    Promoter: CMV,?U6
    Reporter: CD4
    Key Function: Gene Editing,?Stable Cell Line Development,?Genome Engineering,?Episomal Expression
    Product Size: 10 reactions
    Cloning Method: Restriction Enzyme ? MCS
    Delivery Method: Transfection
    Protein Tag or Fusion: CD4 fusion

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    内容及储存

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    Contains:
    ? CRISPR CD4 Nuclease Vector, linearized
    ? 10X Oligonucleotide Annealing Buffer
    ? DNase/RNase-free Water
    ? 5X Ligation Buffer
    ? T4 DNA Ligase
    ? U6 Forward Sequencing Primer
    ? Control Double-stranded Oligonucleotide

    Store in freezer (-5 to -30°C).

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    描述

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    The GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads? CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt? CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease.

    The GeneArt? CRISPR Nuclease Vector system offers:

    ? Easy–to-design genome engineering system
    ? Affordable, ready-to-use cloning vectors
    ? Enrichment for hard to transfect or difficult to modify cell lines

    All-in-one Vector System for CRISPR-based Genome Editing
    The GeneArt? CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA). This system allows you to edit and engineer a genomic locus of choice in a sequence-specific manner from a single plasmid. After relevant targets have been identified with fast and easy-to-use GeneArt? CRISPR vectors, the biologically relevant mutations can be precisely created with GeneArt? Precision TALs, with high specificity and low off-target effects.

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    Copyright??2005?-?2015?北京优健萌威医药科技有限公司
    犀牛云提供企业云服务
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  • 【新时代 新作为 新篇章】增绿植绿 2019-04-19
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