? CRISPR CD4 Nuclease Vector, linearized
? 10X Oligonucleotide Annealing Buffer
? DNase/RNase-free Water
? 5X Ligation Buffer
? T4 DNA Ligase
? U6 Forward Sequencing Primer
? Control Double-stranded Oligonucleotide
Store in freezer (-5 to -30°C).
?The GeneArt? CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR/Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads? CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt? CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease.The GeneArt? CRISPR Nuclease Vector system offers:? Easy–to-design genome engineering system? Affordable, ready-to-use cloning vectors? Enrichment for hard to transfect or difficult to modify cell linesAll-in-one Vector System for CRISPR-based Genome EditingThe GeneArt? CRISPR Nuclease Vector kit offers a simple, ready-to-use, all-in-one expression vector system consisting of both a Cas9 nuclease expression cassette and a guide RNA (gRNA) cloning cassette for rapid and efficient cloning of DNA that encodes target-specific CRISPR RNA (crRNA). This system allows you to edit and engineer a genomic locus of choice in a sequence-specific manner from a single plasmid. After relevant targets have been identified with fast and easy-to-use GeneArt? CRISPR vectors, the biologically relevant mutations can be precisely created with GeneArt? Precision TALs, with high specificity and low off-target effects.